PureFocus™850 Laser Autofocus System


Hardware Laser Autofocus For Industrial And Life Science Microscopy

PureFocus850 combines advanced optics and intelligent microprocessing to provide a real-time focus system for infinity-corrected optical systems. It offers sample detection, dynamic power adjustment, and long focus recapture range.

A motorized offset lens allows adjustment of the imaging depth into the sample, continuously holding the precise distance between the imaging focal point and a reference interface of choice or enabling different discrete image planes to be acquired using the same interface. 

Mounted in the infinity space, it is compatible with most brands of commercial microscopes as well as Prior's OpenStand imaging platform, Queensgate piezo devices, and can be adapted for third-party focus actuators. See product compatibility tab for details.

Designed for versatility, PureFocus850 is suitable for applications where it can detect a reflective surface. As well as metallurgical, geological, and semiconductor samples, it can be used for life science applications using slides, glass bottom dishes, and flow chambers.  

The laser's 850 nm operating wavelength does not interfere with common wavelengths used for illumination and imaging. 

Users can choose to operate the PureFocus850 as a standalone device or integrate it with control software as part of a comprehensive automated imaging system.


    • Rapid automated focus recovery for numerous applications
    • Integrated stepper motor driver and interface to Piezo and other actuators via 0-10 V analog output
    • Easily adapted to any infinity optical system, both upright and inverted
    • Stores and recalls multiple objective-specific parameters for multi-objective use
    • Customizable optics for specific imaging applications
Product Code PF850 PF850M
Dichroic clear aperture, diameter 32 mm 32 mm
Laser optical output < 0.77 mW < 0.77 mW
Laser class Class 1 Class 1
Laser wavelength 850 nm 850 nm
Focus accuracy 20 % of DOF 20 % of DOF
Surface tracking method Point on surface tracking Averaged surface tracking
Typical update rate 1 kHz 1 kHz
Transmitted wavelength range 365 - 800 nm 365 - 800 nm
Capture speed (5x, 0.13NA) < 30 ms < 30 ms
Capture speed (10x, 0.4NA) < 50 ms < 50 ms
Capture speed (20x, 0.5NA) < 60 ms < 60 ms
Capture speed (40x, 0.95NA) < 100 ms < 100 ms
Capture speed (100x, 0.9NA) < 1500 ms < 1500 ms
Capture range (5x, 0.13NA) +/- 15000 μm +/- 15000 μm
Capture range (10x, 0.4NA) +/- 3000 μm +/- 3000 μm
Capture range (20x, 0.5NA) +/- 1000 μm +/- 1000 μm
Capture range (40x, 0.95NA) +/- 500 μm +/- 500 μm
Capture range (100x, 0.9NA) +/- 100 μm +/- 100 μm
Laser module mass 1.5 kg 1.5 kg

Specifications as per datasheet. Please see datasheet for details.

  • PF209 PureFocus Setup Sample Slide

  • PF300 PureFocus Setup Camera

  • PF200 PureFocus Setup Target (RMS, W26, M32 threads)

  • PF201 PureFocus Setup Target (M25, M27, M32 threads)

  • PF404 Piezo Cable for PF850 15D to BNC

Option Manufacturer Compatibility Notes

PF850(M)

+ LF335

Evident/Olympus BX53M, MX50, MX51, MX61, MX61L, MX63, MX63L

PF850(M)

+ LF320

Nikon LV100ND, LV100NDA, LV150N, LV150NA, L200N, L200ND, L300N, L300ND, Ni-U

PF850(M)

+ LF335

Prior OpenStand-U, OpenStand-M, OpenStand-L, OpenStand-V

PF850(M)

+ PF208

Evident/Olympus IX73 (2-deck) Contact Prior Scientific if both decks are occupied

PF850(M)

+ PF214

Evident/Olympus IX73 (1-deck)

PF850(M)

+ PF203

Evident/Olympus IX71

PF850(M)

+ PF213

Evident/Olympus IX81

PF850(M)

+ PF211

Nikon Ti-U

PF850(M)

+ PF212

Nikon Ti2-U, Ti2-A Contact Prior Scientific for Ti2-E

PF850(M)

+ PF210

Evident/Olympus BX51, BX53

PF850(M)

+ LF341

Zeiss AxioSkop 40 Please check with Prior Scientific for Axioimager models

PF850(M)

+ LF312

Leica DM4000, DM5000, DM6000, DM6

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